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Two‐dimensional gel electrophoresis and immunoblotting of human serum albumin modified by reaction with penicillins
Author(s) -
Marzocchi Barbara,
Magi Barbara,
Bini Luca,
Cellesi Carla,
Rossolini Aldo,
Massidda Orietta,
Pallini Vitaliano
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601140
Subject(s) - chromatography , gel electrophoresis , albumin , two dimensional gel electrophoresis , electrophoresis , serum albumin , chemistry , in vitro , human serum albumin , gel electrophoresis of proteins , human albumin , polyacrylamide gel electrophoresis , biochemistry , enzyme , proteomics , gene
A two‐dimensional gel electrophoresis and immunoblotting procedure has been developed to assess the level of modification by penicillins in human serum albumin. The procedure can be used in in vitro experiments and in clinical studies with sera from patients treated with penicillins.