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A novel strategy to identify maternal and paternal inheritance in the mouse
Author(s) -
Römer Irmgard,
Jungblut Peter,
Reik Wolf,
Otto Albrecht,
Klose Joachim
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601137
Subject(s) - inheritance (genetic algorithm) , biology , genetics , evolutionary biology , computational biology , non mendelian inheritance , genealogy , gene , history , mitochondrial dna
A novel strategy for identifying proteins which reveal maternal or paternal inheritance in the mouse is presented. Using two‐dimensional electrophoresis we investigated protein expression patterns of adult liver and different embryonic and extraembryonic tissue in C57BL/6Crl and in DBA/2Crl mice, as well as in their reciprocal hybrids. We found three groups of protein spots which showed maternal or paternal inheritance of quantitative variations. These proteins were characterized by N ‐terminal or internal amino acid sequencing, by determination of the amino acid composition, by glycoprotein staining and RNA expression analysis. The three proteins identified were: α‐enolase, cyclophilin and β‐group hemoglobins. The parental effects observed for α‐enolase and cyclophilin were found to be due to parent‐specific post‐translational modifications of these proteins. For the β‐group hemoglobins our results suggested parental effects on the transcriptional level.