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Detection of platinum species in plant material
Author(s) -
Messerschmidt Jürgen,
Alt Friedrich,
Tölg Günther
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601131
Subject(s) - platinum , isotachophoresis , chromatography , chemistry , ultrafiltration (renal) , detection limit , voltammetry , gel electrophoresis , analytical chemistry (journal) , electrolyte , electrochemistry , biochemistry , electrode , catalysis
Model experiments for the detection of platinum species in extracts from native and platinum‐treated grass cultivations are described. The procedural steps are cultivation of the grass samples, extraction and concentration of the platinum species by ultrafiltration and freeze‐drying, preparative separation of the species by gel chromatography followed by isotachophoresis, and sequential analytical detection of the separated platinum species by adsorptive voltammetry. After isotachophoresis, sharp peaks of platinum species could be detected. In the native grass extract only one platinum species (160–200 kDa) was found. In the platinum‐treated grass extracts several platinum species were observed in the molecular mass range from 1 to > 1000 kDa. By an extremely sensitive platinum determination method (adsorptive voltammetry; detection limit, 2 pg Pt abs.) it was possible to detect platinum even in stained protein bands from horizontal gel electrophoresis of platinum containing fractions obtained after isotachophoresis.