Premium
Effective blotting of ultrathin polyacrylamide gels anchored to a solid matrix
Author(s) -
Heukeshoven Jochen,
Dernick Rudolf
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601123
Subject(s) - polyacrylamide , isoelectric focusing , blot , polyacrylamide gel electrophoresis , chromatography , electrophoresis , chemistry , materials science , biochemistry , polymer chemistry , gene , enzyme
Ultrathin polyacrylamide gels bound on glass plates or plastic sheets cannot be removed from their support without destruction. Therefore electrophoretic transfer methods are not applicable. We have developed a fast diffusion blotting procedure which is very simple and does not need any equipment like blotting chamber or power supply. Furthermore, no special buffer solutions are required. The method is universally applicable to ultrathin sodium dodecyl sulfate, native as well as isoelectric focusing polyacrylamide gels Parts of this investigation were presented at ICES‐ELPHO 93, Sandefjord, Norway, June 2–4 1993 .