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Molecular characterization of Borrelia burgdorferi sensu lato strains by pulsed‐field gel electrophoresis
Author(s) -
Busch Ulrich,
Teufel Cecilia Hizo,
Boehmer Reinhard,
Wilske Bettina,
PreacMursic Vera
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601122
Subject(s) - borrelia burgdorferi , sensu , gel electrophoresis , borrelia , pulsed field gel electrophoresis , biology , characterization (materials science) , microbiology and biotechnology , materials science , genetics , zoology , nanotechnology , antibody , gene , genotype , genus
The large restriction fragment patterns (LRFP) and linear plasmid profiles of eight tick isolates of Borrelia burgdorferi sensu lato were investigated with pulsed‐field gel electrophoresis (PFGE). The whole cell lysate was examined with sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE). The Mlu I LRFP differentiates two species of Borrelia burgdorferi sensu lato ( Borrelia burgdorferi sensu stricto and Borrelia garinii ). LRFP is a suitable method to demonstrate genetic hetero‐ or homogeneity of isolates within one species without subsequent hybridization utilizing diverse probes. Different strains with similar or identical LRFP can be further discriminated by plasmid profile analysis. Our results show that each strain analyzed had a different plasmid profile. Therefore the linear plasmid profile has a potential application as a strain typing procedure. SDS‐PAGE of whole‐cell lysate supports the findings of homology within the B. burgdorferi sensu stricto species and the heterology within the B. garinii species.