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Detection of two hypervariable (ATTTT)n loci in the human genome
Author(s) -
Dürr Christine,
Hinney Anke,
Luckenbach Christine,
Ritter Horst
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601116
Subject(s) - locus (genetics) , genetics , biology , mendelian inheritance , southern blot , polymerase chain reaction , allele , oligonucleotide , dna , microsatellite , gene , microbiology and biotechnology
Object of this investigation was the isolation of a single‐locus probe from a multi‐locus fingerprint. Individual specific multi‐locus fingerprints in man were generated by using the oligonucleotide probe (ATTTT)5. An isolated (ATTTT)5‐positive DNA fragment was analyzed using polymerase chain reaction (PCR) cycle‐sequencing and nonradioactive direct‐blotting electrophoresis. A digoxigenated oligonucleotide synthesized according to this sequence was used as a single‐locus probe. Two hypervariable loci were detected on Southern blots. Formal genetic investigations for the two loci were performed in order to estimate the allele frequencies. Locus 1 shows an individual‐specific banding pattern with an autosomal‐codominant inheritance and can be used for forensic investigations. Locus 2 also represents a polymorphic pattern, but the inheritance is not according to the Mendelian rules. Probably we have detected a highly mutagenic locus in the human genome.