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High‐performance capillary electrophoretic separation of proteins and peptides using a bonded hydrophilic phase capillary
Author(s) -
Piccoli Giovanni,
Fiorani Mara,
Biagiarelli Beatrice,
Palma Francesco,
Vallorani Luciana,
De Bellis Roberta,
Stocchi Vilberto
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601100
Subject(s) - capillary electrophoresis , capillary action , chromatography , capillary electrochromatography , chemistry , silanol , electrophoresis , capillary electrophoresis–mass spectrometry , phase (matter) , peptide , mass spectrometry , materials science , biochemistry , electrospray ionization , organic chemistry , composite material , catalysis
High performance capillary electrophoresis (HPCE) was applied to the separation of protein and peptide mixtures with molecular masses ranging from 1300 to 96000 Da using a new bonded hydrophilic phase capillary, CElect‐P150. This coated capillary reduces the interaction between proteins and silanol groups in capillary walls, allowing a complete recovery of the proteins and peptides of interest. HPCE was also used for the analysis of a complex mixture of tryptic fragments and to monitor the process of enzymatic digestion. Moreover, using a CElect‐P150 capillary, highly reproducible analysis was possible without preconditioning the capillary with acid or basic solutions before each new analysis.