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Alzheimer brain proteins investigated by two‐dimensional gel electrophoresis with immobilized pH gradients in the first dimension
Author(s) -
Mattila Kari M.,
Frey Harry
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150150199
Subject(s) - electrophoresis , spots , chemistry , chromatography , gel electrophoresis , two dimensional gel electrophoresis , immobilized ph gradient , biophysics , biochemistry , enzyme , biology , proteomics , isoelectric focusing , gene
Abstract Two‐dimensional (2‐D) gel electrophoresis with immobilized pH gradient 4–8 in the first dimension was applied in the analysis of Alzheimer's disease brain proteins. The silver‐stained 2‐D maps of extracts from the frontal cerebral cortex were examined. About 800 and 550 protein spots could be observed on the electrophoretograms from the total and buffer‐soluble fractions, respectively. In comparing the gels, four protein spots could be seen in all Alzheimer 2‐D patterns which were either undetectable (one spot) or significantly weaker (two spots) or stronger (one spot) in density than those from the controls.