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The homology of a novel polypeptide with stress protein characteristics in embryonic mice brain and in the hypothalamus of caloric restricted rats as determined by ultramicro western blotting
Author(s) -
Pipkin James L.,
Hinson William G.,
LynCook Lascelles E.,
La Borde James,
Anson Jeanne F.,
Feuers Ritchie J.,
Duffy Peter H.,
Hart Ronald W.,
Casciano Daniel A.
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150150196
Subject(s) - hypothalamus , blot , homology (biology) , caloric theory , biology , embryonic stem cell , endocrinology , medicine , microbiology and biotechnology , biochemistry , amino acid , gene
A novel protein (p34) was observed in polyacrylamide gel fluorographs of gestation day 13 embryonic mouse brain following retinoic acid dosing of dams. Another p34 polypeptide with identical gel migratory characteristics was seen in the hypothalamus of old caloric restricted rats after “food deprivation stress”. Western blotting, employing an ultramicro trans‐blot cell developed in our laboratory, detected identical immunochemical determinants between these proteins, verifying their homology. Peptide mapping and Western blotting further validated the uniqueness of p34 compared with other stress proteins including heme oxygenase.