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A rapid method for detection of N ‐acetylglucosaminidase‐type chitinase activity in crossed immunoelectrophoresis and sodium dodecyl sulfate‐polyacrylamide gel electrophoresis gels using 4‐methylumbelliferyl‐ N ‐acetyl‐ D ‐glucosaminide as substrate
Author(s) -
Chen KunShang,
Lee KuoKau,
Chen HsingChen
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150150193
Subject(s) - chitinase , sodium dodecyl sulfate , chemistry , polyacrylamide gel electrophoresis , chromatography , gel electrophoresis , substrate (aquarium) , electrophoresis , sodium , polyacrylamide , biochemistry , enzyme , biology , organic chemistry , polymer chemistry , ecology
A method for the detection of N ‐acetylglucosaminidase (GlcNAcase) activity has been developed by using 4‐methyl‐umbelliferyl‐ N ‐acetyl‐ D ‐glucosaminide (4‐MU‐GlcNAc) as substrate in crossed immunoelectrophoresis (CIE) and sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) gels. Visualization of the reaction with a 366 nm ultra‐violet light is possible in approximately 30 min. The method is fast and sensitive in comparison with previous methods. The same band as in SDS‐PAGE, showing both GlcNAcase and chitinase activity, was found in the present study; we therefore conclude that this method is also useful in a GlcNAcase‐type chitinase assay.