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Genomic fingerprinting of Yersinia enterocolitica species by degenerate oligonucleotide‐primed polymerase chain reaction
Author(s) -
Sayada Chalom,
Picard Bertrand,
Elion Jacques,
Krishnamoorthy Rajagopal
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150150176
Subject(s) - yersinia enterocolitica , polymerase chain reaction , biology , primer (cosmetics) , yersinia , genomic dna , microbiology and biotechnology , oligonucleotide , dna profiling , serotype , dna , genetics , bacteria , gene , chemistry , organic chemistry
A novel version of the polymerase chain reaction (PCR) termed degenerate oligonucleotide‐primed PCR (DOP‐PCR) was used to fingerprint the bacterial genome of Yersinia enterocolitica species. Eight well‐characterized reference strains of Y. enterocolitica (ribotype, serotype, biotype and zymotype) were examined. Optimal experimental conditions for reproducibility, sensitivity and specificity were obtained using a single DOP‐primer. All the strains gave distinct DOP‐PCR profiles composed of 12 to 19 fragments with sizes from 200 to 1.500 bp. Thus, the DOP‐PCR, which has so far been used to fingerprint human, mouse, fruitfly and plant DNA, is also well‐suited to bacterial DNA.