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Characterization of gene expression in clinical lung cancer materials by two‐dimensional polyacrylamide gel electrophoresis
Author(s) -
Okuzawa Ken,
Franzén Bo,
Lindholm Johan,
Linder Stig,
Hirano Takashi,
Bergman Thomas,
Ebihara Yoshiro,
Kato Harubumi,
Auer Gert
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150150157
Subject(s) - polyacrylamide gel electrophoresis , gel electrophoresis , lung cancer , characterization (materials science) , microbiology and biotechnology , electrophoresis , chromatography , chemistry , biology , materials science , medicine , biochemistry , pathology , nanotechnology , enzyme
Fourteen human lung tumors of various histopathological types were subjected to two‐dimensional gel electrophoresis (2‐DE). Samples were prepared for 2‐DE using a nonenzymatic sample preparation (NESP) technique recently established in our laboratory. Variations in the expression of some polypeptides were observed between tumors of different histopathological types. To this end, high expression of β‐tubulin, heat shock proteins 73 and 90, lamin B, and proliferating cell nuclear antigen (PCNA) were observed in small cell lung carcinomas (SCLC). One polypeptide of unknown identity (35 kDa, p I 5.5) was significantly overexpressed in primary lung adenocarcinomas compared with SCLC, squamous cell lung carcinomas, metastatic lung adenocarcinomas from colon and rectum, and normal tissue. The amino acid composition of this polypeptide is presented. In summary, combining the NESP technique and 2‐DE is an effective approach to define tumor‐specific markers.