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Expression of tumor‐associated protein variants in chemically induced rat hepatomas and transformed rat liver cell lines determined by two‐dimensional electrophoresis
Author(s) -
ZeindlEberhart Evelyn,
Jungblut Peter,
Rabes Hartmut M.
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150150156
Subject(s) - proteolysis , biology , cell culture , microbiology and biotechnology , amino acid , gel electrophoresis , biochemistry , peptide , cell , liver cell , genetics , enzyme , medicine
High resolution two‐dimensional electrophoresis (2‐DE), using the rat liver as a model, was applied to study hepatocarcinogenesis induced by different chemical carcinogens. Several tumor‐associated protein variants were detected by 2‐DE in chemically induced rat hepatomas and transformed rat liver cell lines compared to normal rat liver tissue. Proliferation‐related protein changes and/or protein alterations due to culture conditions were corrected for by comparison with 2‐DE patterns of isolated cells from regenerating liver and short‐term cultivated liver cells. Some of the tumor‐associated variants were further characterized: (i) By peptide mapping with limited proteolysis we detected clear relationships between several variants. (ii) By studying posttranslational modifications phosphorylated and glycosylated variants could be determined. (iii) A tumor‐associated protein variant was identified by amino acid analysis and amino acid sequencing.