Premium
Towards new formulations for polyacrylamide matrices: N ‐acryloylaminoethoxyethanol, a novel monomer combining high hydrophilicity with extreme hydrolytic stability
Author(s) -
Chiari Marcella,
Micheletti Claudia,
Nesi Marina,
Fazio Marco,
Righetti Pier Giorgio
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150150131
Subject(s) - hydrolysis , monomer , acrylamide , chemistry , isoelectric point , polyacrylamide , polymer , acrylate , electrokinetic phenomena , alkaline hydrolysis , polymer chemistry , chromatography , organic chemistry , enzyme
Matrices for electrokinetic separations, based on a unique class of mono‐ and disubstituted (on the amido nitrogen) acrylamides such as e.g., N ‐acryloylaminoethoxyethanol (AAEE) and acrylamido‐ N,N ‐diethoxyethanol, offer the following advantages: (i) strong resistance to alkaline hydrolysis (most zone separations occurring at basic pH values), (ii) high hydrophilicity and (iii) greater porosity, due to the higher molecular weight of the monomers. When compared with conventional poly(acrylamide), a poly(AAEE) matrix, when subjected to mild alkaline hydrolysis (0.1 N NaOH, 70°C) appears to be 500 times more stable. Such stability is also confirmed under strong alkaline hydrolysis (1 N NaOH, 100°C) as well as under mild and strong acidic hydrolysis. Mildly hydrolyzed poly(AAEE) matrices still perform extremely well in both conventional isoelectric focusing and immobilized pH gradients, techniques which are quite sensitive to traces of acrylate in the polymer coil. Conversely, mildly hydrolyzed poly(acrylamide) matrices, when used in isoelectric focusing, generate pH gradients between pH 4 and 5, having an inflection point (pH 4.6) equivalent to the p K value of acrylic acid. This novel class of monomers shows great promise for future applications in all electrokinetic methodologies.