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Horizontal polyacrylamide gel electrophoresis for the separation of DNA fragments
Author(s) -
Haas Hildegard,
Budowle Bruce,
Weiler Günter
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150150126
Subject(s) - polyacrylamide gel electrophoresis , gel electrophoresis of nucleic acids , chromatography , polyacrylamide , buffer (optical fiber) , chemistry , formate , gel electrophoresis , electrophoresis , dna , monomer , gel electrophoresis of proteins , resolution (logic) , color marker , biochemistry , polymer chemistry , polymer , organic chemistry , telecommunications , artificial intelligence , computer science , enzyme , catalysis
A discontinuous borate/formate buffer system is presented for horizontal polyacrylamide gel electrophoresis of DNA fragments. The resolution potential of the system could be altered by changing the total monomer concentration (5–9%T), the concentration of the crosslinker piperazine diacrylamide (2–5%C PDA ), as well as the concentration of formate in the gel (40–120 m M ), the leading ion of the buffer system. The separation of DNA fragments would be improved by increasing the migration distance from 22 to 28 cm. This discontinuous polyacrylamide gel electrophoresis system proved highly reproducible.