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An algorithm for unwarping multitrack electrophoretic gels
Author(s) -
Glasbey Chris A.,
Wright Frank
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150150124
Subject(s) - electrophoresis , image warping , algorithm , artificial intelligence , computer science , chemistry , pattern recognition (psychology) , biological system , chromatography , biology
An algorithm is proposed for removing the effect of “frowns” and “smiles” ( i.e. warping) from multitrack electrophoretic gels, so that the bands are horizontal and in alignment between tracks. It has been applied to a DNA sequencing autoradiograph, but could equally be applied to other types of gel separation where warping occurs. The algorithm uses a gradient filter to estimate band orientations. These are smoothed and interpolated by robust regression, and integrated to find the unwarping transform. No manual intervention is required.