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Electric birefringence imaging of DNA in agarose electrophoresis gels: Noise limits and prolonged electric field effects
Author(s) -
Lanan Maureen,
Grossmann Daniel W.,
Morris Michael D.
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150150123
Subject(s) - electric field , electrophoresis , agarose , birefringence , agarose gel electrophoresis , dna , noise (video) , materials science , gel electrophoresis of nucleic acids , chemistry , chromatography , optics , physics , biochemistry , computer science , image (mathematics) , quantum mechanics , artificial intelligence
Electric birefringence imaging (EBI) maps stresses in agarose electrophoresis gels. Both DNA and gel alignment can be monitored using this technique. Previously, we have reported birefringence images associated with resolved small (< 48 kbp) and partially resolved large 48 kb – (2 Mbp) DNA fragments oriented with relatively short electric field pulses. In this paper, differences between images acquired using short and long (DC) voltage pulses are presented. It is shown that stress signals distribute over long distances when exposed to prolonged electric fields. Resolution also affects band shapes. Refinements in instrumental design and evaluation of limiting noise sources of the technique are also described.