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Protein studies of human non‐Hodgkin's B‐lymphoma: Appraisal by two‐dimensional gel electrophoresis
Author(s) -
Mohammad Ramzi M.,
Maki Auday,
Vistisen Kerry,
AlKatib Ayad
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501501225
Subject(s) - lymphoma , microbiology and biotechnology , cell culture , polyacrylamide gel electrophoresis , epstein–barr virus , biology , gel electrophoresis , non hodgkin's lymphoma , b cell , flow cytometry , silver stain , antibody , virus , immunology , genetics , biochemistry , enzyme
We have utilized two‐dimensional polyacrylamide gel electrophoresis (2‐D PAGE) coupled with silver stain to identify cellular proteins in human non‐Hodgkin's B‐lymphoma (NHL). Five cell lines (SKDHL2B, WSU‐DLCL2, WSU‐NHL, WSU‐FSCCL and SKLN1), representing four different NHL maturational stages and a normal Epstein‐Barr virus (EBV)‐transformed line of B‐cell origin (SKLN1) were studied. The NHL lines were immunophenotyped using flow cytometry with lineage associated monoclonal antibodies. Whole cell lysates of the cell lines were subjected to 2‐D PAGE analyses. The gels were analyzed with an image scanning computer and the qualitative differences of protein patterns were studied. Results revealed great similarities in patterns of the NHL lines. A master map containing common NHL‐protein spots was constructed. When the map of each tumor line was compared to the master map, several protein spots were associated with each NHL‐grade. Search for these proteins in the normal EBV‐transformed B‐cell line showed that only one of the proteins (S3; M r /p I 19/5.9) was present. Proteins that were detected in malignant NHL, but not in the normal EBV‐line, could provide important information regarding the human NHL B‐lymphocyte databases. Whether or not these proteins are definite malignant markers to distinguish between different NHL maturational stages needs further exploration through electroblotting and microsequencing.