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Consequences of a maximum existing in the dependence of separation selectivity on concentration of cyclodextrin added as chiral selector in capillary zone electrophoresis
Author(s) -
Vespalec Radim,
Fanali Salvatore,
Boček Petr
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501501218
Subject(s) - cyclodextrin , enantiomer , selectivity , capillary electrophoresis , chemistry , chromatography , resolution (logic) , analytical chemistry (journal) , stereochemistry , organic chemistry , artificial intelligence , computer science , catalysis
The maximum in the dependence of the separation selectivity on the concentration of cyclodextrin may be utilized for the determination of the mean value of host‐guest interaction constants of the separated enantiomers. For the hosting of N ‐ t ‐BOC‐ DL ‐tryptophan by β‐cyclodextrin, the mean value of these constants, found by drop line estimated from the cyclodextrin concentration corresponding at the maximum, is 350 L.mol −1 . The separation at this concentration of cyclodextrin offers the highest resolution in the shortest separation time. It is shown that commonly used simple preliminary experiments, testing the capability of a cyclodextrin to resolve chiral compounds, and based on relatively high cyclodextrin concentrations, may easily lead to incorrect conclusions in cases of enantiomers strongly interacting with the cyclodextrin used.