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The human myocardial two‐dimensional gel protein database: Update 1994
Author(s) -
Corbett Joseph M.,
Wheeler Colin H.,
Baker Cathy S.,
Yacoub Magdi H.,
Dunn Michael J.
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501501209
Subject(s) - database , protein expression , human heart , gel electrophoresis , electrophoresis , biology , microbiology and biotechnology , chemistry , genetics , medicine , gene , computer science
An updated human heart protein two‐dimensional electrophoresis (2‐DE) database is presented. The database, which contains some 1388 protein spots characterised in terms of M r and p I , has been analysed further by Western immunoblotting and protein sequencing. From a total of 103 protein spots analysed, 49 have been identified by immunoblotting and 32 have been identified by protein sequencing. A further six proteins have tentatively been assigned by comparison with the human heart 2‐DE protein database of Jungblut et al. (Electrophoresis ) 1994, 15 , 685–607). This database is being used in studies of alterations in protein expression in the diseased and transplanted human heart.