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Analysis of lower aliphatic aldehydes in water by micellar electrokinetic chromatography with derivatization to 2,4‐dinitrophenylhydrazones
Author(s) -
Takeda Sahori,
Wakida Shinichi,
Yamane Masataka,
Higashi Kunishige
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501501203
Subject(s) - micellar electrokinetic chromatography , derivatization , chemistry , chromatography , acetaldehyde , reagent , high performance liquid chromatography , formaldehyde , acetonitrile , electrokinetic phenomena , reproducibility , detection limit , organic chemistry , ethanol
The analysis of lower aliphatic aldehydes in water following derivatization to 2,4‐dinitrophenylhydrazones, was investigated by micellar electrokinetic chromatography (MEKC). The effects of the buffer pH and acetonitrile addition on the separation were investigated. Under optimized conditions the 2,4‐dinitrophenylhydrazine (DNPH) reagent was completely separated from four DNPH derivatized aldehydes with high separation efficiency. DNPH‐formaldehyde and DNPH‐acetaldehyde were also completely separated from one another. Their relative standard deviation of migration times and peak areas were within 1.0% and 3.3%, respectively. The reproducibility of peak areas in this MEKC analysis was as good as that of high‐performance liquid chromatography (HPLC) analysis with the same derivatization method. The recovery values from tap and river water were in the range of 97–102%. The detection limits of formaldehyde and acetaldehyde were 0.05 mg/L and 0.08 mg/L respectively.