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Determination of benzodiazepines by micellar electrokinetic chromatography
Author(s) -
Bechet Isabelle,
Fillet Marianne,
Hubert Philippe,
Crommen Jacques
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501501200
Subject(s) - triethanolamine , micellar electrokinetic chromatography , chemistry , chromatography , oxazepam , sodium dodecyl sulfate , methanol , pulmonary surfactant , electrokinetic phenomena , aqueous solution , glycine , resolution (logic) , detection limit , analytical chemistry (journal) , organic chemistry , amino acid , biochemistry , receptor , artificial intelligence , computer science , benzodiazepine
A method for the separation and determination of benzodiazepines by micellar electrokinetic chromatography (MEKC) has been developed. Separation buffers consisted of aqueous solutions of glycine and triethanolamine (pH 9.0), containing sodium dodecyl sulfate (SDS) as surfactant and methanol as organic modifier. The effect of the concentration of SDS, methanol, glycine and triethanolamine on migration times and resolution was studied. Ten benzodiazepines were baseline separated at a 25 m M SDS concentration and 20% v/v methanol in a 75m M glycine‐250 m M triethanolamine buffer. Under these conditions, the within‐day reproducibilities were 0.3–0.5% for migration times and 1.7–1.9% for peak areas at a concentration of 10 μg/mL. The limits of detection and quantification for oxazepam were 0.2 and 0.7 μg/mL, respectively, using an injection time of 5s.