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Micellar electrokinetic chromatography of the anti‐human immunodeficiency virus (HIV) agent michellamine B with absorption and laser‐induced fluorescence detection
Author(s) -
Chan King C.,
Majadly Fathy,
McCloud Thomas G.,
Muschik Gary M.,
Issaq Haleem J.,
Snader Kenneth M.
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501501199
Subject(s) - micellar electrokinetic chromatography , sodium dodecyl sulfate , chromatography , chemistry , detection limit , fluorescence , sodium , sodium cholate , acetonitrile , laser induced fluorescence , micelle , absorption (acoustics) , high performance liquid chromatography , buffer solution , fluorescence spectroscopy , aqueous solution , materials science , organic chemistry , physics , quantum mechanics , composite material
Micellar electrokinetic chromatography (MEKC) was applied to the separation of the anti‐HIV agents, michellamines A and B, and two other structurally related monomers found in the extract of the Ancistrocladus plants. Using buffers containing either 10 m M sodium phosphate (pH 7.0), 50 m M sodium de‐oxycholate and 10–20% acetonitrile or 5 m M sodium phosphate (pH 7.0), 20 m M sodium dodecyl sulfate and 25% acetonitrile allowed baseline separations of the four components in the mixture in less than 10 min. The MEKC methods gave sharper peaks and better resolution compared to high‐performance liquid chromatography. For MEKC separation of the plant extracts, UV absorption detection provided adequate sensitivity; however, higher sensitivity could be achieved with UV laser‐induced fluorescence detection (LIF). Using the sodium dodecyl sulfate‐containing buffer and LIF, the limit of detection for michellamine B was ∼ 2 ng/mL. The sensitivity was degraded ∼100‐fold when using the deoxycholate buffer because of high background fluorescence. Preliminary results show that MEKC with LIF is feasible for the sensitive detection of michellamine B in serum.