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Application of micellar electrokinetic chromatography to the quality control of pharmaceutical formulations: The analysis of xanthine derivatives
Author(s) -
Korman Milos,
Vindevogel Johan,
Sandra Pat
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501501198
Subject(s) - micellar electrokinetic chromatography , chromatography , chemistry , theophylline , caffeine , xanthine , sodium dodecyl sulfate , pulmonary surfactant , ephedrine , detection limit , pharmacology , organic chemistry , medicine , biochemistry , enzyme , endocrinology
Micellar electrokinetic chromatography (MEKC) has been applied to the analysis of three different drugs. Although belonging to different therapeutic classes, all these drugs contain xanthine derivatives as active substances. Pen‐toxifylline was separated from eight related xanthines. Quantitative MEKC was applied to determine impurities (caffeine and xanthine) in the purified drug at the 0.05–0.1% level and also for the determination of the active substance in Agapurin® tablets. Ethofylline and theophylline were separated from ephedrine and mebrophenhydramine and determined in Xantedrylettae® tablets while caffeine was separated from mephenhydramine and determined in Kinedryl® tablets. In all cases, simple borate buffers with sodium dodecyl sulfate as the surfactant were satisfactory and little separation optimization was required. The relative standard deviation (RSD) of the migration times was better than 1% while the RSD of the observed areas was better than 2%. This demonstrates that MEKC is a valuable alternative for the traditional high‐performance liquid chromatography analysis of drugs and drug formulations.