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Effect of organic modifier on resolution of hydrophobic compounds by micellar electrokinetic chromatography
Author(s) -
Kaneta Takashi,
Yamashita Tetsuya,
Imasaka Totaro
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501501193
Subject(s) - micellar electrokinetic chromatography , acetonitrile , dimethylformamide , chemistry , capacity factor , methanol , resolution (logic) , electrokinetic phenomena , chromatography , hydrophobic effect , high performance liquid chromatography , organic chemistry , electrophoresis , artificial intelligence , solvent , computer science
Three organic modifiers, methanol, acetonitrile, and dimethylformamide, were used as organic modifiers to improve the resolution of hydrophobic compounds in micellar electrokinetic chromatography. The capacity factor decreased with increasing concentration of organic modifier. Acetonitrile and dimethylformamide were more effective modifiers to decrease the capacity factor than methanol. Maximum resolution was obtained when the capacity factor was reduced to about 2. Methanol enhanced the resolution for less hydrophobic compounds, while acetonitrile and dimethylformamide were better modifiers to improve the resolution for strongly hydrophobic compounds.