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A large scale preparative gel electrophoresis separation of α 1 and α 2 subunits of the voltage‐gated Ca 2+ channel from rabbit skeletal muscle
Author(s) -
Nyormoi Okot,
Schneider Toni,
Smith Robert G.
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501501178
Subject(s) - skeletal muscle , electrophoresis , chemistry , protein subunit , resolution (logic) , alpha (finance) , gel electrophoresis , molar ratio , elution , chromatography , analytical chemistry (journal) , biochemistry , biology , anatomy , medicine , construct validity , nursing , artificial intelligence , computer science , patient satisfaction , gene , catalysis
A large‐scale preparative gel electrophoresis method effectively separates individual voltage‐gated calcium channel (VGCC) subunits. with high resolution, starting with up to 4 mg of rabbit skeletal muscle L‐type VGCC complex. Using this method, we separated α 1 and α 2 subunits of rabbit skeletal muscle VGCC with a high efficiency and with protein recoveries of 83%. The separated α 1 and α 2 subunits eluted from the gel in a 1:1 molar ratio. The method should be applicable for separating the other VGCC subunits or subunits of other protein complexes.