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Capillary zone electrophoresis of oligosaccharides derivatized with various aminonaphthalene sulfonic acids
Author(s) -
Chiesa Claudia,
O'Neill Roger A.
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501501171
Subject(s) - capillary electrophoresis , chemistry , electrophoresis , resolution (logic) , chromatography , reductive amination , analyte , electrolyte , organic chemistry , electrode , artificial intelligence , computer science , catalysis
Malto‐oligosaccharides were derivatized via their reducing ends with different aminonaphthalene mono‐, di‐ and trisulfonic acids by reductive amination. The derivatives were then separated by capillary zone eléctrophoresis in uncoated fused silica capillaries, using 50 m M triethylammonium phosphate buffer, pH 2.5, as running electrolyte. The effect of degree of charge on speed of analysis and resolution was studied for different aminonaphthalene mono‐, di‐ and trisulfonic acids. Under the conditions used, a higher degree of charge on the derivatives provided both faster analyses and higher resolution. Investigation of the electrophoretic behavior of derivatized oligosaccharides obtained from bovine pancreatic ribonuclease B gave insight into the possibility of applying such electrophoretic systems to the analysis of more complex carbohydrates. The resolution of positional isomers under the conditions described indicated that the high resolving power of this technique allows separations not strictly based on the effects of charge and mass of the analytes, but on structural characteristics as well. The relationship between electrophoretic mobility and molecular structure was investigated for the different derivatives.