Focused antigen capture immunoassay | Zendy

Suen Kitty K. Y. | Zendy; Lewis W. H. P. | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Focused antigen capture immunoassay

Author(s) -

Suen Kitty K. Y.,

Lewis W. H. P.

Publication year - 1994

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.11501501169

Subject(s) - nitrocellulose , isoelectric focusing , immunoassay , densitometry , chromatography , coefficient of variation , antigen , antibody , chemistry , microbiology and biotechnology , membrane , biology , immunology , biochemistry , medicine , enzyme

A new approach is described to immunoblotting in which antigens after isoelectric focusing are captured on nitrocellulose membranes coated with a specific antibody. The method has been applied to the analysis of human IgA ϰ and λ from whole blood and serum without prior purification. The visualized bands were quantified by laser densitometry in four p I ranges. It is concluded that, under the conditions used, close to 100% of serum IgA is captured. The method has a coefficient of variation of 4.5–12.8%, mean 8.0%.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research