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Separation of chlorophyll‐protein complexes by deriphat polyacrylamide gradient gel electrophoresis
Author(s) -
Sárvári Éva,
Nyitrai Péter
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501501159
Subject(s) - photosystem ii , polyacrylamide gel electrophoresis , chromatography , acrylamide , polyacrylamide , chemistry , photosystem i , electrophoresis , resolution (logic) , gel electrophoresis , chlorophyll , biochemistry , photosynthesis , organic chemistry , monomer , polymer chemistry , enzyme , artificial intelligence , computer science , polymer
An improved Deriphat polyacrylamide gradient gel electrophoresis system was developed for the separation of chlorophyll‐protein complexes. The relatively good resolution of the starting discontinuous gel system was further improved by using glycerol in gels and an acrylamide gradient with high acrylamide‐to‐ N,N ′,‐methylenebisacrylamide ratio in the separating gel. By applying mild but efficient glycosidic detergents for solubilization, and Deriphat to gels and buffers, the stability of complexes was increased, and only a low amount of pigment was removed. The advantage of our system is the better resolution of larger‐size complexes, especially those of photosystem I. In addition, it makes possible an easier interpretation of results due to less overlapping of photosystem I and photosystem II bands when different plant species or the effects of different treatments are compared using whole thylakoid membranes.