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Application of micellar electrokinetic capillary chromatography for monitoring of hippuric and methylhippuric acid in human urine
Author(s) -
Lee KongJoo,
Lee Jong Jin,
Moon Dong Cheul
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150150115
Subject(s) - micellar electrokinetic chromatography , chromatography , urine , chemistry , hippuric acid , electrokinetic phenomena , capillary action , capillary electrophoresis , materials science , biochemistry , composite material
The factors affecting micellar electrokinetic capillary chromatographic separation of hippuric and o ‐, m ‐, p ‐methylhippuric acid were investigated by changing the species of micelles, and adding urea to the micellar solution. The analysis of hippurates in human urine is demonstrated under optimum conditions using 20 m M phosphate buffer (pH 8.0) containing 100 m M dodecyltrimethylammonium bromide and 4 M urea at −22 kV applied voltage. This method proved suitable for the screening of hippurates in human urine following occupational exposure to toluene and xylene.