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Preparative isoelectric focusing in multicompartment electrolyzers: Novel, hydrolytically stable and hydrophilic isoelectric membranes
Author(s) -
Chiari Marcella,
Nesi Marina,
Roncada Paola,
Righetti Pier Giorgio
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501501139
Subject(s) - membrane , isoelectric point , isoelectric focusing , chromatography , chemistry , polyacrylamide , hydrolysis , acrylamide , monomer , acrylic acid , polymer chemistry , polymer , organic chemistry , biochemistry , enzyme
Preparative isoelectric focusing in multicompartment electrolyzers is based on the production of isoelectric membranes of precise isoelectric point, able to buffer at their p I value and to titrate proteins tangent to or crossing the membranes. Up to the present, such membranes have been based on polyacrylamide chemistry; acrylamide, however, is neither stable in acidic nor basic environments. We describe here novel membranes, produced with a unique monomer, N ‐acryloylaminoethoxyethanol (AAEE). Poly(AAEE) membranes are extremely stable to alkaline hydrolysis (500 times more stable than polyacrylamide) and even more hydrophilic than the latter matrix. This allows production of highly reproducible membranes (these do not change their p I with time, since no acrylic acid is produced by hydrolysis upon storage) which do not adsorb proteins by hydrophobic interaction.