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Method for rapid electrophoretic transfer of isoelectric focusing gels to polyvinylidene difluoride
Author(s) -
Lobert Sharon,
Correia John J.
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501501135
Subject(s) - isoelectric focusing , chromatography , chemistry , electrophoresis , sodium dodecyl sulfate , isoelectric point , biochemistry , enzyme
We present here a protocol for unidirectional wet electrotransfer of isoelectric focusing (IEF) gels to polyvinylidene difluoride (PVDF) that can be carried out in < 2 h, including preequilibration steps. In developing this protocol, we systematically evaluated sodium dodecyl sulfate and methanol concentrations in the preequilibration washes and length of electrotransfer. We demonstrated that the method is useful for tubulin, a protein that resists usual semi‐dry transfer procedures due to its limited solubility under IEF conditions. We also successfully used our method for transferring the microtubule‐associated protein, tau, as well as carbamylyte standards, creatine phosphokinase and carbonic anhydrase.