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Potential and limitations of an optically active crown ether for chiral separation in capillary zone electrophoresis
Author(s) -
Kuhn Reinhard,
Wagner Joseph,
Walbroehl Yvonne,
Bereuter Thomas
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501501117
Subject(s) - capillary electrophoresis , optically active , crown ether , chromatography , capillary action , separation (statistics) , chemistry , electrophoresis , capillary electrochromatography , materials science , organic chemistry , computer science , ion , machine learning , composite material
Capillary zone electrophoresis using optically active 18‐crown‐6 tetracarboxylic acid (18C6H 4 ) as chiral selector was studied for the enantiomeric separation of primary amines. From the separation of a variety of pharmaceutical drug substances, amino alcohols and amino acids, conclusions could be made concerning the influence of the chemical structure of the analytes on the separation. In addition, the effects of experimental parameters such as pH, proportion of organic modifier and buffer composition on the separation are discussed. A synergistic effect obtained by the joint application of 18C6H 4 and a cyclodextrin was exploited to resolve analytes which were separated neither by the crown ether nor by the cyclodextrin.