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Neutral and anionic cyclodextrins in capillary zone electrophoresis: Enantiomeric separation of ephedrine and related compounds
Author(s) -
Dette Christoph,
Ebel Siegfried,
Terabe Shigeru
Publication year - 1994
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501501112
Subject(s) - capillary electrophoresis , chemistry , ephedrine , enantiomer , cyclodextrin , chromatography , reagent , phosphate buffered saline , sodium , derivative (finance) , beta cyclodextrins , electrophoresis , organic chemistry , economics , biology , neuroscience , financial economics
Two methods for the enantiomeric separation of chiral ephedrine alkaloids (ephedrine, methylephedrine, methylpseudoephedrine and norephedrine) by capillary zone electrophoresis in uncoated capillaries were developed. Both methods were optimized to more than 100 000 theoretical plate numbers. The first method used a neutral cyclodextrin (CD) derivative: heptakis (2,6–di‐ O ‐methyl)‐β‐cyclodextrin at an acidic pH of 2.5 (20 m M phosphate buffer) at an 18 m M concentration. The second used a newly developed acidic CD derivative, the tetrakis[6‐ O ‐(4‐sulfobutyl)]‐β‐cyclodextrin sodium salt. The benefits of this new reagent for the chiral separation are a wide range of basic pH available for the enantiomeric resolution. It is also useful for other cases, e.g. preventing adsorption without additives.