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A three‐dimensional plot for the display of transverse pore gradient gel electrophoresis data: Application to a kinetoplast DNA fragment of planar circular conformation
Author(s) -
Wheeler David,
Chrambach Andreas
Publication year - 1993
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150140190
Subject(s) - kinetoplast , dna , gel electrophoresis , crithidia fasciculata , transverse plane , electrophoresis , circular dna , polyacrylamide gel electrophoresis , chemistry , biophysics , chromatography , crystallography , biology , biochemistry , anatomy , genome , gene , enzyme
A 219 bp fragment of kinetoplast DNA from Crithidia fasciculata has previously been shown to exhibit a gel concentration dependent, anomalously low migration rate in 3–10% polyacrylamide transverse pore gradient gels relative to that of straight standards. The anomaly was interpreted in accordance with previous results of Marini et al. [1] as being due to the planar circular conformation of the fragment. The transverse pore gradient gel pattern is displayed here as a three‐dimensional plot of migration distance vs. gel concentration and DNA length for the kinetoplast fragment and straight DNA standards. The plot yields a smooth surface for the standards clearly separated from the curve of the kinetoplast DNA fragment. Thus, transverse gradient gel electrophoresis in conjunction with the three‐dimensional plot provides a unique method for the recognition of abnormal conformational features of DNA on the basis of electrophoretic mobility in a pore‐size gradient.