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Application of high‐performance capillary electrophoresis to the analysis of H1 histones
Author(s) -
Lindner Herbert,
Wurm Martin,
Dirschlmayer Arnold,
Sarg Bettina,
Helliger Wilfried
Publication year - 1993
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150140174
Subject(s) - capillary electrophoresis , chromatography , histone , electrophoresis , buffer (optical fiber) , phosphate buffered saline , chemistry , capillary action , high performance liquid chromatography , gel electrophoresis , histone h1 , cellulose , dna , biochemistry , materials science , telecommunications , computer science , composite material
High‐performance capillary electrophoresis for the separation of rat testis H1 histone variants and their phosphorylated modifications is described. The influence of buffer pH, hydroxypropylmethyl cellulose, and buffer concentration has been investigated. Under optimized conditions (500 m M phosphate buffer, pH 2, 0.03% hydroxypropylmethyl cellulose) using an uncoated capillary, eight H1 histone subfractions, including two H1° histones and H1t and their phosphorylated modifications, are resolved. Application of capillary electrophoresis to the separation of H1 histones provides an important new alternative to high‐performance liquid chromatography (HPLC) and traditional gel electrophoresis.