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High‐performance capillary electrophoresis of glycoconjugates
Author(s) -
Novotny Milos V.,
Sudor Jan
Publication year - 1993
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150140163
Subject(s) - glycoconjugate , capillary electrophoresis , monosaccharide , glycobiology , derivatization , biomolecule , chemistry , affinity electrophoresis , chromatography , mass spectrometry , glycan , biochemistry , glycoprotein , affinity chromatography , enzyme
Capillary electrophoresis (CE) has recently emerged as a highly promising technique for the analysis and structural study of glycoconjugates. This article reviews the current status of CE and its potential in glycobiology. The separation media suitable for the analysis of monosaccharides, oligosaccharides and very large carbohydrate‐based biomolecules are discussed. The detection aspects emphasize chemical derivatization approaches to tagging the glycoconjugates of interest for enhanced response in absorption and fluorescence spectroscopy.