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Progress in developing improved programs for pulsed field agarose gel electrophoresis of DNA
Author(s) -
Arshad Malik F.,
Dunn Frederick J.,
Vega Raul,
Valvano Jonathan W.,
Serwer Philip
Publication year - 1993
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150140158
Subject(s) - pulsed field gel electrophoresis , agarose , gel electrophoresis , gel electrophoresis of nucleic acids , agarose gel electrophoresis , polarity (international relations) , schizosaccharomyces pombe , electrophoresis , resolution (logic) , dna , materials science , chromatography , chemistry , biology , microbiology and biotechnology , genetics , computer science , gene , artificial intelligence , genotype , cell , mutant
Details are described here for using a rotating gel to perform pulsed field agarose gel electrophoresis (PFGE) with programmable control of the following variables: magnitude of the electrical field, polarity of the electrical field, temperature of the gel and position of the rotating disk upon which the agarose gel rests. By use of this procedure for programmable control, modes of PFGE have been explored that have the following characteristics: (i) resolution by DNA length is completely lost for DNA shorter than a critical length that increases as the pulse times increase, and (ii) resolution by DNA length is enhanced for longer DNAs that are shorter than a second critical length. This window of resolution can be moved to the position of the 2–6 Mb chromosomes of Schizosaccharomyces pombe .