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Study of pH‐dependent structural transition in telomeric sequences by two‐dimensional gel electrophoresis
Author(s) -
Belotserkovskii Boris P.,
FrankKamenetskii Maxim D.
Publication year - 1993
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150140147
Subject(s) - electrophoresis , transition (genetics) , gel electrophoresis , chemistry , two dimensional gel electrophoresis , chromatography , temperature gradient gel electrophoresis , biochemistry , gene , proteomics , 16s ribosomal rna
We have applied the method of two‐dimensional gel electrophoresis to study a pH‐dependent structural transition in plasmid carrying the Tetrahymena telomeric sequence, (G 4 T 2 ) n . A study of the two‐dimensional patterns makes it possible to obtain energetic characteristics of the transition. We have obtained the nucleation energy of the structure. It proved to be two times lower than the nucleation energy of H‐DNA determined before for a d(AG) n insert. The finding partly explains the formation of an eclectic structure in telomeric sequences. We also studied the effect of Zn ++ ions on the two‐dimensional patterns of gel electrophoresis. Our data show that Zn ++ ions can significantly affect the transitions themselves. This finding leads to reinterpretation of numerous data on probing of structural transitions in DNA by the S1 nuclease.