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Electrophoretic analysis of stimulated cat parotid saliva
Author(s) -
Marshall Tom,
Williams Katherine M.,
Ekström Jürgen,
Tobin Gunnar,
Bayard Cecilia,
Vesterberg Olof
Publication year - 1993
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501401204
Subject(s) - saliva , electrophoresis , chemistry , chromatography , biochemistry
The proteins of parasympathetically stimulated cat parotid saliva were analyzed by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and high resolution two‐dimensional electrophoresis (2‐DE). SDS‐PAGE revealed up to 30 polypeptide bands in microliter volumes of unconcentrated saliva. The patterns were highly reproducible and characterized by prominent bands of M r 57000, M r 30000 and M r 15000. The major protein ( M r 30000) appeared as a dimer ( M r 60000) when electrophoresed under non‐reducing conditions but dissociated into its monomeric form when the SDS concentration of the denatured samples was increased from 1 to 5%. This indicates a noncovalent association. The protein patterns of saliva from different cats differed slightly but sequential samples from the same cat (collected during 90 min of stimulation) showed little change in protein pattern apart from a fall in total protein content. Following 2‐DE, the major protein ( M r 30000) appeared as a complex array of at least eight spots in two tiers (p I 5.2–6.2; M r 28000 and 32000). The characteristics of this protein are discussed with reference to allergy to cats.