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Salivary deoxyribounuclease I polymorphism separated by polyacrylamide gel‐isoelectric focusing and detected by the dried agarose film overlay method
Author(s) -
Tenjo Etsuko,
Sawazaki Kazumi,
Yasuda Toshihiro,
Nadano Daita,
Takeshita Haruo,
Iida Reiko,
Kishi Koichiro
Publication year - 1993
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501401166
Subject(s) - isoelectric focusing , agarose , chromatography , polyacrylamide , polyacrylamide gel electrophoresis , chemistry , materials science , microbiology and biotechnology , biology , biochemistry , enzyme
Isoelectric focusing of whole saliva samples on polyacrylamide gels (pH 3.5–5), followed by dried agarose film overlay detection, was employed to determine the type of salivary deoxyribonuclease I (DNase I). Since this detection method had not only a high sensitivity, but also a high band resolution, it was possible to determine DNase I types from saliva samples of 2–5 μL. Pretreatment of saliva samples with neuraminidase simplified the isozyme pattern and enhanced the sensitivity. The DNase I types in all 30 saliva samples showed a good correlation with the types found in the corresponding blood, semen, and urine samples. This preliminary trial indicates that DNase I typing from saliva samples is a new and promising method for individualization of casework samples in the field of forensic biology.