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Immunoblotting of Yersinia plasmid‐encoded released proteins: A tool for serodiagnosis
Author(s) -
Cremer Josef,
Putzker Michael,
Faulde Michael,
Zöller Lothar
Publication year - 1993
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501401151
Subject(s) - yersinia enterocolitica , serotype , antigen , plasmid , yersiniosis , microbiology and biotechnology , yersinia infections , gel electrophoresis , yersinia , enterobacteriaceae , biology , antibody , sodium dodecyl sulfate , chemistry , escherichia coli , bacteria , gene , biochemistry , immunology , genetics
The plasmid‐encoded, released proteins (RPs) of Yersinia enterocolitica seroypes 09 and 03 were separated by sodium dodecyl sulfate (SDS)‐pore gradient gel electrophoresis. The RP‐patterns of both serotypes proved to be identical. Five major proteins of M r 27000, 34700, 35600, 45800, and 46800 were detected. Spontaneously plasmid‐cured derivatives of the two serotypes lost the feature of protein release. By immunoblotting of RP with sera from patients suffering from acute Yersinia infections, specific and reproducible band patterns were obtained. Laser scan densitometry was applied to record the immunoreactions quantitatively. Predominant bands were detected at an M r of 34700 and 35600. IgA and IgM antibodies appeared as acute‐phase markers rapidly decreasing in the reconvalescent phase. In contrast, immunoblots of patients with supposed chronic yersiniosis were characterized by a persisting IgA and elevated IgG reactivity. The application of RP as diagnostic antigens proved to be advantageous because they are naturally separated from cross‐reacting proteins, common to pathogenic and nonpathogenic strains of Yersinia enterocolitica and Y. pseudotuberculosis .