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Analysis of trp repressor‐DNA interactions using gel electrophoresis
Author(s) -
Lewis D. E. A.,
Carey Jannette
Publication year - 1993
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501401113
Subject(s) - repressor , gel electrophoresis , dna , electrophoretic mobility shift assay , electrophoresis , temperature gradient gel electrophoresis , chemistry , microbiology and biotechnology , genetics , biology , biochemistry , gene , transcription factor , 16s ribosomal rna
Quantitative analysis of the DNA‐binding equilibria of E. coli trp repressor by gel electrophoresis led to reevaluation of our understanding of this complex system. In this review, the data leading to controversy about the trp system are discussed, and our current understanding is presented.