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A simple physical model for the gel electrophoretic manifestations of sequence‐dependent DNA superstructures
Author(s) -
De Santis Pasquale,
Palleschi Antonio,
Savino Maria
Publication year - 1993
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501401111
Subject(s) - electrophoresis , dna , oligonucleotide , curvature , base pair , sequence (biology) , gel electrophoresis of nucleic acids , chemistry , biophysics , biological system , materials science , biology , chromatography , biochemistry , mathematics , geometry
Abstract A simple physical model of the gel electrophoretic manifestations of sequence‐dependent DNA superstructures is illustrated. The model is based on the calculation of the curvature dispersion as evaluated by integrating the orientational parameters of the base pairs along the chain. Such a quantity is proportional to the straightening activation energy of the curved DNA in the direction of the average orientation of the helical axis and was found to be proportional to the logarithm of the gel electrophoretic retardation. The model is capable of consistently explaining the different electrophoretic manifestations of DNA superstructures, such as the retardation of a large ensemble of multimeric oligonucleotides with different sequences, periodicities, and lengths, and the permutation gel assays as well as the mobility changes consequent to extensive point mutations in a DNA tract.