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The silver staining procedure of sodium dodecyl sulfate‐gels may be accelerated by shortening fixation time
Author(s) -
Kirkeby Svend,
Moe Dennis,
BògHansen Thorkild C.
Publication year - 1993
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150140109
Subject(s) - glutaraldehyde , staining , chemistry , sodium dodecyl sulfate , chromatography , silver stain , acetic acid , picric acid , fixation (population genetics) , ethanol , formaldehyde , sodium , gel electrophoresis , polyacrylamide gel electrophoresis , biochemistry , microbiology and biotechnology , biology , organic chemistry , enzyme , genetics , gene
In most silver staining methods the first step in the staining of proteins separated after sodium dodecyl sulfate‐polyacrylamide gel electrophoresis is a rather protracted fixation of the gels. Optimum fixation should be short, cause no background staining and effectively immobilize the proteins in the gel without masking the proteins for reaction with the staining solution. Further, the concentration of the fixing compounds should be as low as possible due to the potentional toxicity of fixatives. Fixation for only 5 min with mixtures of very low concentrations of formaldehyde and glutaraldehyde in ethanol, or a solution of formaldehyde or glutaraldehyde in picric acid and ethanol, fulfill these demands, provided that the gels were prefixed in ethanol‐acetic acid for 10 min. As a consequence of these results a fast and sensitive silver staining procedure is proposed.