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Sex identification by polymerase chain reaction of α‐satellite in aged tissue samples
Author(s) -
Fattorini Paolo,
Cacció Simone,
Gustincih Stefano,
Florian Fiorella,
Altamura Bruno M.,
Graziosi Giorgio
Publication year - 1993
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150140105
Subject(s) - polymerase chain reaction , stain , biology , microbiology and biotechnology , genomic dna , satellite dna , satellite , dna , blood stains , saliva , genetics , staining , chemistry , chromosome , gene , chromatography , biochemistry , engineering , aerospace engineering
In vitro amplification of the alphoid repeated sequences clustered in the centromeric regions of the human × and Y chromosomes was carried out. A modification of the amplification conditions reported by Witt and Erickson [1] gave clear amplification bands from 10 pg of genomic DNA template. Results were obtained from the following aged tissue samples: 1 μL three‐year‐old blood stain, one‐year‐old single hair roots, six‐month‐old saliva stains and twelve‐year‐old bone fragments. Good results were obtained even when the template was artificially fragmented into fragments of less than 1 kbp.
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