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Relative efficiency of molecular sieving in solutions of four polymers
Author(s) -
Chrambach Andreas,
Aldroubi Akram
Publication year - 1993
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150140104
Subject(s) - polymer , radius , polystyrene , polyvinyl alcohol , particle size , polyacrylamide , chemistry , analytical chemistry (journal) , cellulose , range (aeronautics) , agarose , particle (ecology) , materials science , chromatography , polymer chemistry , organic chemistry , composite material , oceanography , computer security , computer science , geology
The efficiency of size separations of polystyrene sulfate, 120‐1085 nm radius, by molecular sieving in polymer solutions, expressed by the separation efficiency function S = M O d K R ( R )/d R T e − K R ( R ) T [where M O is the mobility in free solution, K R the retardation coefficient, R the geometric mean radius of the particle, K R ( R ) the retardation coefficient as a function of R , and the polymer concentration is T ] increases from methylhydroxypropyl cellulose to polyvinyl alcohol to uncrosslinked polyacrylamide to agarose above its gelling temperature. Separations of DNA, in the size range of 3–21 nm radius, are by at least one order of magnitude more efficient than those of polystyrene sulfate in the size range of 120–1085 nm radius. A plot of S vs. R in the experimental range of T is constructed for the four polymer solutions; this allows one to select optimal media and concentrations for the sieving of particles in the desired range of molecular sizes.