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A comparison between low background silver diammine and silver nitrate protein stains
Author(s) -
Rabilloud Thierry
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130190
Subject(s) - silver nitrate , silver stain , polyacrylamide , chemistry , polyacrylamide gel electrophoresis , glutaraldehyde , chromatography , sodium dodecyl sulfate , staining , matrix (chemical analysis) , nuclear chemistry , biochemistry , biology , microbiology and biotechnology , polymer chemistry , enzyme , genetics
Several methods of silver staining of proteins after sodium dodecyl sulfate‐electrophoresis in polyacrylamide gels were compared. The most rapid methods were found to be less sensitive than more time‐consuming methods. Among the long methods, those using glutaraldehyde treatment of the gel and silver diammine complex as the silvering agent were found to be the most sensitive, at the expense of the use of a modified polyacrylamide matrix and higher silver concentrations.