Calculation of restriction fragment lengths by image processing | Zendy

Holmlund Gunilla | Zendy; Karlberg Knut | Zendy; Gustavsson Bengt | Zendy; Lindblom Bertil | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Calculation of restriction fragment lengths by image processing

Author(s) -

Holmlund Gunilla,

Karlberg Knut,

Gustavsson Bengt,

Lindblom Bertil

Publication year - 1992

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150130186

Subject(s) - fragment (logic) , reciprocal , image (mathematics) , image processing , mathematics , algorithm , physics , computer science , artificial intelligence , philosophy , linguistics

The image processing system, LabEye Profile, calculates the size of DNA restriction fragments according to the formula MD = f( x ); x = 1/log bp. The migration distances ( MD ) of known fragment sizes are interpolated by the use of cubic splines. Cubic splines are also used to correct for gel distortions. A comparison to the Biotrac system, which uses the reciprocal method, gives almost identical standard deviations for calculated sizes compared to known sizes, 0.88% for LabEye Profile and 0.89% for Biotrac. The measurement error for LabEye Profile is 0.24% of the size of the fragments.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research