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Calculation of restriction fragment lengths by image processing
Author(s) -
Holmlund Gunilla,
Karlberg Knut,
Gustavsson Bengt,
Lindblom Bertil
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130186
Subject(s) - fragment (logic) , reciprocal , image (mathematics) , image processing , mathematics , algorithm , physics , computer science , artificial intelligence , philosophy , linguistics
The image processing system, LabEye Profile, calculates the size of DNA restriction fragments according to the formula MD = f( x ); x = 1/log bp. The migration distances ( MD ) of known fragment sizes are interpolated by the use of cubic splines. Cubic splines are also used to correct for gel distortions. A comparison to the Biotrac system, which uses the reciprocal method, gives almost identical standard deviations for calculated sizes compared to known sizes, 0.88% for LabEye Profile and 0.89% for Biotrac. The measurement error for LabEye Profile is 0.24% of the size of the fragments.