A new technique for detecting oxytocinase activity in electrophoresis gels | Zendy

Roy Ashim C. | Zendy; Saha Nilmani | Zendy; Tan Suan M. | Zendy; Kamarul Farida Z. | Zendy; Ratnam Shan S. | Zendy

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A new technique for detecting oxytocinase activity in electrophoresis gels

Author(s) -

Roy Ashim C.,

Saha Nilmani,

Tan Suan M.,

Kamarul Farida Z.,

Ratnam Shan S.

Publication year - 1992

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150130181

Subject(s) - chemistry , chromatography , magenta , sodium nitrite , monomer , electrophoresis , sodium , organic chemistry , materials science , inkwell , composite material , polymer

A sensitive staining method for the detection of oxytocinase (EC 3.4.11.3) activity in electrophoresis gels has been described. The method is based on the enzymatic release of p ‐nitroaniline (PNA) from two specific synthetic oxytocinase sub‐strates, S‐benzyl‐ L ‐cysteine‐ p ‐nitroanilide (BCN) and L ‐leucine‐ p ‐nitroanilide (LN), respectively. The PNA is then diazotized with sodium nitrite and subsequently coupled to a chromogen, N ‐(1‐naphtyl)‐ethylenediamine dihydrochloride (NED) to produce a deep pink/magenta colored azo‐dye at the site of oxytocinase activity.

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