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A new technique for detecting oxytocinase activity in electrophoresis gels
Author(s) -
Roy Ashim C.,
Saha Nilmani,
Tan Suan M.,
Kamarul Farida Z.,
Ratnam Shan S.
Publication year - 1992
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150130181
Subject(s) - chemistry , chromatography , magenta , sodium nitrite , monomer , electrophoresis , sodium , organic chemistry , materials science , inkwell , composite material , polymer
A sensitive staining method for the detection of oxytocinase (EC 3.4.11.3) activity in electrophoresis gels has been described. The method is based on the enzymatic release of p ‐nitroaniline (PNA) from two specific synthetic oxytocinase sub‐strates, S‐benzyl‐ L ‐cysteine‐ p ‐nitroanilide (BCN) and L ‐leucine‐ p ‐nitroanilide (LN), respectively. The PNA is then diazotized with sodium nitrite and subsequently coupled to a chromogen, N ‐(1‐naphtyl)‐ethylenediamine dihydrochloride (NED) to produce a deep pink/magenta colored azo‐dye at the site of oxytocinase activity.